The oligomeric Rep protein of Mungbean yellow mosaic India virus (MYMIV) is a likely replicative helicase
نویسندگان
چکیده
Geminiviruses replicate by rolling circle mode of replication (RCR) and the viral Rep protein initiates RCR by the site-specific nicking at a conserved nonamer (TAATATT downward arrow AC) sequence. The mechanism of subsequent steps of the replication process, e.g. helicase activity to drive fork-elongation, etc. has largely remained obscure. Here we show that Rep of a geminivirus, namely, Mungbean yellow mosaic India virus (MYMIV), acts as a replicative helicase. The Rep-helicase, requiring > or =6 nt space for its efficient activity, translocates in the 3'-->5' direction, and the presence of forked junction in the substrate does not influence the activity to any great extent. Rep forms a large oligomeric complex and the helicase activity is dependent on the oligomeric conformation ( approximately 24mer). The role of Rep as a replicative helicase has been demonstrated through ex vivo studies in Saccharomyces cerevisiae and in planta analyses in Nicotiana tabacum. We also establish that such helicase activity is not confined to the MYMIV system alone, but is also true with at least two other begomoviruses, viz., Mungbean yellow mosaic virus (MYMV) and Indian cassava mosaic virus (ICMV).
منابع مشابه
Yellow mosaic virus infecting soybean in northern India is distinct from the species infecting soybean in southern and western India
Genomic components of the begomovirus causing yellow mosaic disease (YMD) in soybean in Delhi were cloned, sequenced and infectivity proved. Nucleotide sequence analysis of the virus isolate revealed more than 89% identity with Mungbean yellow mosaic India virus (MYMIV) and therefore it is designated as soybean isolate of MYMIV (MYMIV-[Sb]). Total nucleotide and predicted amino acid sequence an...
متن کاملDNA replication and Pathogenecity of MYMIV
Geminiviridae is a large family of single stranded DNA plant viruses, transmitted by white fly Bamesia tabacci, and they are the major causes of huge agro-economical losses worldwide. Mungbean yellow mosaic India virus (MYMIV) belongs to the genus begomovirus, the predominant variety of geminiviruses in northern part of Indian subcontinent. It has bipartite genome, which replicates via rolling ...
متن کاملDetection of quantitative trait loci for mungbean yellow mosaic India virus (MYMIV) resistance in mungbean (Vigna radiata (L.) Wilczek) in India and Pakistan
Yellow mosaic disease (YMD) is one of the major diseases affecting mungbean (Vigna radiata (L.) Wilczek). In this study, we report the mapping of the quantitative trait locus (QTL) for mungbean yellow mosaic India virus (MYMIV) resistance in mungbean. An F8 recombinant inbred line (RIL) mapping population was generated in Thailand from a cross between NM10-12-1 (MYMIV resistance) and KPS2 (MYMI...
متن کاملMapping of Mungbean Yellow Mosaic India Virus (MYMIV) and powdery mildew resistant gene in black gram [Vigna mungo(L.) Hepper]
Black gram, one of the important species of Asian Vigna group of grain legumes, is widely grown in South Asia and is an important source of dietary protein. The two main biological constraints particularly Mungbean Yellow Mosaic India Virus(MYMIV) and powdery mildew pose a major threat to black gram production in India. Several reports on mapping mungbean yellow mosaic virus disease and powdery...
متن کاملLinkage mapping of Mungbean yellow mosaic India virus (MYMIV) resistance gene in soybean
Mungbean Yellow Mosaic India Virus (MYMIV) is one of the most prevalent pathogen that limits soybean production in India. In this study RILs derived from JS335, dominant but MYMIV susceptible variety and PI171443, donor of MYMIV resistance gene in most of the MYMIV resistant varieties released in India and F2 population derived from SL525, a resistant variety released for northern India and NRC...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Nucleic Acids Research
دوره 34 شماره
صفحات -
تاریخ انتشار 2006